LOCUS       40924_29521        5545 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pLYS1K DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pLYS1K
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5545)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..956
                     /pseudo
                     /gene="lys1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..956
                     /pseudo
                     /codon_start=1
                     /gene="lys1"
                     /label=lys1
                     /note="NotI site is created at the middle of this fragment 
                     (613-620)."
                     /translation="VLCSTCAVSRSNIFGIAKRCHSSRTWHEECPTFGDQSF*Y*QDMR
                     YRRSW*NISSCWWIG*GLPWK**ID*QKILKELVC*SLQVCRPNSRKCALETLLVRNTR
                     SYVSFW*LGPLSPNW*CRM*RPPTIKLKFVVSELS*VKLIPIFLVILMSVKILPLFDVI
                     RMKNPR*LHTLYLKV*IKMTLIALLSRKILSSMV*RSTGSLYMIYGNILRLNFLVMLFL
                     L*LFPFIKCLSILMER"
     gene            242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
     gene            998..2354
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(2405..2421)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2634..3089
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3371..3475
                     /label=AmpR promoter
     CDS             3476..4333
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4507..5095
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5383..5404
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5419..5449
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5457..5473
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5481..5497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtactttgt agtacctgcg cggtctcaag atcaaacatt tttggaatcg caaaaagatg
      301 tcattccagc cggacgtggc atgaagaatg tccaactttt ggtgatcaat cgttttgata
      361 ctaacaagat atgcggtatc ggagaagttg gtgaaatata tcttcgtgct ggtggattgg
      421 ctgagggcta ccttggaaat gatgaattga ctagcaaaaa attcttaaag agttggtttg
      481 ctgatccctc caagtttgta gaccgaactc cagaaaatgc gccctggaaa ccttattggt
      541 tcggaatacg cgatcgtatg tatcgttctg gtgacttggg ccgctatctc ccaactggta
      601 atgtagaatg tagcggccgc cgacgatcaa attaaaattc gtggtttccg aattgagtta
      661 ggtgaaatta atacccatct ttctcgtcat cctaatgtcc gtgaaaatat tacccttgtt
      721 cgacgtgata aggatgaaga acccacgcta gttgcataca ttgtacctca aggtttaaat
      781 aaagatgact ttgatagcgc tactgagtcg gaagatattg tcgtcaatgg tttgaagaag
      841 tacaggaagc ttatacatga tatacgggaa tatcttaaga ctaaacttcc tagttatgct
      901 attccttctg taattgttcc ccttcataaa atgcctctca atcctaatgg aaagatctgt
      961 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     1021 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     1081 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1141 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1201 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1261 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1321 cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta
     1381 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1441 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1501 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1561 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1621 ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat
     1681 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1741 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1801 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1861 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     1921 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1981 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2041 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2101 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg
     2161 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     2221 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     2281 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     2341 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga
     2401 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2461 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2521 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2581 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     2641 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2701 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2761 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2821 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2881 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2941 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     3001 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3061 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     3121 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3181 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3241 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3301 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3481 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3541 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3601 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3661 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3721 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3781 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3841 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3901 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3961 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4021 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4081 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4141 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4201 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4261 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4321 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4381 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4621 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5221 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5281 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     5341 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     5401 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     5461 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg
     5521 cctgcaggtc gactctagag gatcc
//
